| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Manuel, Tie B (16)
|
MAGRA |
22.89 |
22.91 |
0.02 |
| 2 |
Thompson, Will C (16)
|
MAMSA |
24.63 |
24.59 |
-0.04 |
| 3 |
Arjona, Alexander J (15)
|
MANSC |
25.20 |
24.73 |
-0.47 |
| 4 |
Lesher, Charlie M (15)
|
MAGRA |
24.50 |
24.84 |
0.34 |
| 5 |
Hendrickson, Brady (15)
|
MALCY |
|
24.91 |
- |
| 6 |
Humphrey, Zach D (17)
|
MAGRA |
24.21 |
25.00 |
0.79 |
| 7 |
Tangorra, Beppe L (15)
|
MARKRY |
26.13 |
25.03 |
-1.10 |
| 8 |
Overman, Adam T (15)
|
MAFWAC |
25.11 |
25.07 |
-0.04 |
| 9 |
Muthaiyan, Pranav D (16)
|
MAWA |
24.74 |
25.30 |
0.56 |
| 10 |
Zabigalivk, Maksym (16)
|
MANSC |
25.42 |
25.39 |
-0.03 |
| 11 |
Tebo, Tyler V (16)
|
MAGRA |
24.45 |
25.43 |
0.98 |
| 12 |
DeHaven, Regan J (16)
|
MAWA |
25.27 |
25.87 |
0.60 |
| 13 |
Miller, Steven R (16)
|
MACAA |
26.94 |
26.96 |
0.02 |
| 14 |
McKnight, Grant T (15)
|
MAGRA |
27.24 |
27.18 |
-0.06 |
| 15 |
Srinivasan, Siddarth (15)
|
MAWA |
29.05 |
27.36 |
-1.69 |
| 16 |
Cohn, Joshua B (16)
|
MAACAC |
27.93 |
28.01 |
0.08 |
| 17 |
Bherwal, Vansh (15)
|
MALCY |
33.88 |
34.12 |
0.24 |
|
McLellan, Matthew (15)
|
MAWA |
25.51 |
NS |
- |
|
Gorman, Ronan M (18)
|
MAGRA |
23.05 |
NS |
- |
|
Agamalian, Zachary M (15)
|
MAACAC |
28.21 |
NS |
- |